system of Escherichia coli
نویسنده
چکیده
Dzwokai Wla,̂ '̂ David N. Cook,^ Wlarie Albert!,^ NingG. Pon,^ Hiroshi Nikaido'̂ '* and John E. 'Department of Chemistry, University of Gatlfornia, Berkeley, California 94720. USA. ^Division of Structural Biology. Lawrence Berkeley Laboratory, Berkeley. California 94720. USA. '^Sferitech inc., 2525 Stanwell Drive, Suite 300, Concord, California 94520, USA. '^Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
منابع مشابه
CONSTRUCTION OF RECOMBINANT PLASMIDS FOR PERIPLASMIC EXPRESSION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI UNDER T7 AND LAC PROMOTERS
In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the related cDNA was inserted in two expression plasmids carrying pelB signal peptide, one with lac bacterial promoter and the other with a bacteriophage T7-based promoter. The recombinant plasmids were moved to TG1 and BL21 strains of E. coli, respectively. To induce the expression systems, IPTG and...
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